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Centre for BioMaterials , Cellular and Molecular Theranautics(CBCMT)

Facilities

Cellular and Molecular Therapeutics Laboratory

About the laboratory

Our laboratory mainly focuses on utilizing the different aspects of cellular and molecular therapeutics in finding potential new targets/ways which can contribute to novel alternative treatment options for several degenerative clinical conditions. Currently we are focussing on (but not limited to) the following 3 broad areas: a. Stem cell and gene therapy to target neurodegenerative disorders, brain cancers as well as tissue repair. b. Therapeutic benefit of modulating inflammatory responses to reduce bleed related joint damage in hemophilic arthropathy. c. Understanding the potential role of the opioid system (especially the delta opioids) in neuronal development, survival and differentiation.

Facilities available at the Cellular and Molecular Therapeutics Laboratory

  • BSL-II-A2 Biosafety cabinet
  • CO2 incubators
  • Inverted microscope with high definition camera
  • Refrigerated centrifuges
  • -20°C ultra freezer and liquid nitrogen storage tank, 4°C refrigerator

All kinds of equipment that are required to carry out DNA, RNA, protein work. Example- Gradient PCR machine, Gel electrophoresis units, Immunoblot apparatus, UV spectrophotometer, bacterial incubator shaker, heat block, ice flake machine etc.

Biocorrosion and Biotribology Laboratory

About the laboratory

Corrosion and wear are the major factors that affects the service period of an implant when exposed to the aggressive environment as in human body. Development of new materials and coatings to alleviate wear and corrosion are the major focus of biomaterials research to enhance the longevity of mankind. The major focus of this laboratory is to evaluate and understand the behaviour of widely used implant materials such as titanium and magnesium in simulated body conditions. The corrosion of coated and uncoated substrates are evaluated using ACM Gill potentiostat under controlled environment. A reciprocator wear tester (Ducom) with temperature, pH and environment controlled equipment is utilized to assess wear rate and friction of materials. The scratch resistance of the thin film coatings are measured using either a static or ramp loaded scratch tester provided with 200 µm dia diamond indenter.

A three electrode system containing platinum as auxiliary electrode, Standard calomel electrode as reference electrode and test sample as working electrode is employed for the testing. The test is run in Simulated Body Fluid (SBF) in accordance with ASTM F-2129 standard. The EIS and polarization studies are performed with ASTM G-102 and G-103 standards.

Specifications:

  • Frequency: 10 µHz to 100 KHz
  • Amplitude: 1 to 232 mV
  • Sweep rate: 0.1666-10 mV/sec
  • Sweep Range: ± 3 V

The reciprocatory wear tester was developed by Ducom Industries, Bangalore which was custom-made to study the wear behaviour of coatings in simulated body fluid condition and temperature of 37°C, with the advantage of counter material which can be both a pin or ball made of ceramic material or Ultra High Molecular Weight Polyethylene (UHMWPE). The wear test is run using ASTM G133 standard. The wear rate and co-efficient of friction with respect to number of cycles are obtained as the output from this testing. The wear track can be analyzed for further studies and the wear debris can also be collected and analyzed.

Specifications:

  • Sample dimension: 30 x 25 x 5 mm (LxBxH)
  • Pin/ Ball dimension: 5-6 mm diameter/ 5-6mm height (pin)
  • Sliding distance: 15, 20 and 25 mm stroke length
  • Load: 1-10 N
  • Temperature control: 25-37°C
  • Frequency: 0.1-2 Hz

To evaluate the adhesion strength of coatings Scratch tester developed by Ducom Industries, Bangalore is employed in our laboratory. A diamond indenter with 200 micron tip radius transverse the coating surface and records the tractional load with respect to stroke and the co-efficient of friction can also be obtained as the output. This scratch tester carries a sample stage which can move in 3-axis (xyz) and also has the advantage of two loading transducer systems having range between 0-20 N and 0-200 N respectively. The tests can be carried out in constant and ramp loading modes with the loading rate of 0.2-2 N/mm

Specifications:

  • Sample dimension: 20x25x10mm (LxBxH)
  • Load: 0-20 N/ 0-200 N
  • Scan speed: 0.25-1 mm/sec
  • Loading rate: 0.2-2 N/mm
  • Stroke length: 0.1-25 mm

Material Processing Laboratory

About the laboratory

Key challenges in the field of implantology are stress shielding, low fatigue strength, poor bone bonding and formation of biofilim on implants. This lab focuses on development of materials with tailored mechanical properties by subjecting the material to thermomechanical processing. Materials with grain size ranging from micron and to nano are being investigated to have optimum modulus closer to bone and high fatigue and mechanical strength. Two high temperature furnaces with controlled environment is available to facilitate this work. In addition, novel coatings to improve the osseointegration and prevent bacterial formation are developed using simple cost effective techniques such as Electrophoretic deposition (EPD) and micro arc oxidation (MAO). In addition, this laboratory also involves activities related to replacement and reconstruction of diseased or damaged parts of the musculo-skeletal/dental systems by near net shape manufacturing of implants using different material and biological characterizations. Further, this lab focuses on development of new generation of ceramic, ceramic- polymeric/metallic composites for different biomedical applications. A substantial portion of this lab is involved in colloidal process of ceramics, bio-ceramics, protein coagulation casting, sol-gel and implant designing (dental and maxillofacial prosthesis) for bio-medical applications.

Box furnace
  • Temperature: RT-1200°C / 5-10°C/min control
  • Control: HP Argon gas

Tubular furnace

  • Temperature: RT-900°C / 5-10°C/min control
  • Control: HP Argon gas
  • Double Disk polishing machine
  • Low speed cutting saw (0-100rpm)
  • Carl Zeiss Microscope with Clemex Image analyzer (Magnification: 50x to 500x)
  • Fume cupboard
  • Ultrasonic bath with 3 KHz frequency and 25-50°C heater
  • Electrophoretic Deposition system: 0-130 V DC source/ 0-5 Amps current control
  • Micro Arc Oxidation system: 0-220 V AC source/ Frequency control with Sine, Triangular and Rectangular waveform
  • Double Distillation unit
  • Magnetic stirrer
  • -4°C Freezer
  • Suction pump and Vacuum Decicators
  • Weighing Balance (0.0001 gm)
  • Sessile drop method
  • Fitted with camera
  • Load range: 1000gms
  • Fitted with Camera ,Data acquisition & also can be manually operated

Composite Materials Laboratory

About the laboratory

The laboratory focused on processing and characterization of polymer composites. The main emphasis is on tailoring the interface of polymer and fillers by modification of fillers and an addition of compatibilizer. In addition, we are working on polymer blends and its composites.

Facilities:

  • Twin screw extruder
  • Mini injection molding machine
  • Palletizer
  • Vacuum oven