Centre for Biomaterials, Cellular and Molecular Theranostics (CBCMT)

Drug Discovery Unit (DDU)

The Drug Discovery Unit (DDU) was established in 2020 and is a part of the Centre for Biomaterials, Cellular, and Molecular Theranostics (CBCMT), VIT, Vellore. The core aim of DDU is to find innovative basic science findings and initiate preclinical drug discovery and development, translating them into the bedside. Currently, DDU is actively involved in research on developing small molecule drug design and discovery, fluorescent imaging agents for assisting cancer surgery, developing proteolysis targeting chimera (PROTAC) for cancer therapy, finding small molecules for cancer immunotherapy, developing dual-targeting agents to selectively target cancer cells, developing photosensitizers for cancer therapy, therapeutics agents for various disease such as diabetes, antimicrobial resistance, cardiovascular diseases, tuberculosis, and fibrosis, etc. and developing biotherapeutics such as miRNA, siRNA and protein-based therapeutics.

Lab In-charge: Dr. Loganathan Rangasamy

Email: loganathan.r@vit.ac.in

1. Fume Hood

2. HPLC - Analytical & Preparative System

3. Flash Chromatography System

4. Heidolph Rotary Evaporator

5. Oscillating Hydrogenator

6. Smart Evaporator

7. Hot Air Oven

8. Ultrasonic Cleaner

9. Peptide Shaker (LP – Shaker 360 AMPv)

10. Computational Workstation

Biomaterials Processing and Characterization Facility

Key challenges in the field of implantology are stress shielding, low fatigue strength, poor bone bonding and formation of biofilm on implants. This laboratory has all the basic equipment’s needed for the development of materials with tailored mechanical properties. Materials with grain size ranging from micron and to nano are being investigated to have optimum modulus closer to bone and high fatigue and mechanical strength. Two high temperature furnaces with controlled environment is available to facilitate this work. In addition, novel coatings to improve the osseointegration and prevent bacterial formation are developed using simple cost-effective techniques such as Electrophoretic deposition (EPD) and micro arc oxidation (MAO). In addition, this laboratory also involves activities related to replacement and reconstruction of diseased or damaged parts of the musculo-skeletal/dental systems by near net shape manufacturing of implants using different material and biological characterizations. Further, this lab focuses on development of new generation of ceramic, ceramic- polymeric/metallic composites for different biomedical applications. A substantial portion of this lab is involved in colloidal process of ceramics, bio-ceramics, protein coagulation casting, sol-gel and implant designing (dental and maxillofacial prosthesis) for bio-medical applications.

Lab In-charge: Dr. Loganathan Rangasamy

Email: loganathan.r@vit.ac.in

1. Contact Angle Measuring System

2. Carl Zeiss Microscope

3. Plasma Cleaner

4. High Temperature Box Furnace

5. Tribo-corrosion Tester

6. Tubular Furnace

7. Anodization & Electrophoretic deposition

8. UV- Vis Spectrophotometer

HOME Lab (3D Bioprinting and Imaging Facility)

3D bioprinting facilities are a recent addition to our centre. The advanced 3D bioprinting facility is called HOME (Human Organ Manufacturing and Engineering) Lab, equipped with two high-end Cellink Bio X bioprinter as well as few basic bioprinters. With this addition, the centre is better positioned to fabricate 3D organoids and tissue mimics, which will be also be one of the key focus areas in coming years.

Lab In-charge: Dr. Anjaneyulu U

Email: anjaneyulu.u@vit.ac.in

1. Olympus FLUOVIEW FV3000, Confocal Laser Scanning Microscope

Olympus’ new FLUOVIEW FV3000 confocal laser scanning microscope combines high-performance imaging capabilities with ease of use so researchers in such fields as cell biology, cancer research and stem cell research can collect relevant imaging data quickly and easily.

2. Olympus Inverted Microscope Model CKX53e

The CKX53 microscope eases the cell and tissue culture workflow, simplifying steps such as live cell observation, cell sampling and handling, image capture, and fluorescence observation.

3. EVOS M5000

The fully integrated EVOS M5000 Imaging System combines precision optics, an articulated 18.5-inch high-resolution LCD monitor, and a highly sensitive camera.

4. Lumen- X Gen 3 DLP Bioprinter

LUMEN X™​ Gen 3 brings a new degree of precision and utility to the light-based bioprinter space. Years of listening to our customers’ needs and feedback have culminated in LUMEN X™​ Gen 3, packed with an array of new features and simplified workflows.

5. BIO X 3D Bioprinter

The BIO X bioprinter is the industry standard trusted by researchers, doctors, and industry professionals across the globe, revolutionizing fields such as tissue engineering, drug discovery, toxicity research, and enabling researchers to do more in 3D cell culture faster.

6. DLAB SafeVac Vacuum Aspiration System

Cellular and Molecular Therapeutics Laboratory

Cellular and Molecular Therapeutics Laboratory (CMTL) is a laboratory focusing on developing creative approaches uniting diagnosis and therapy for various diseases, particularly those with significant unmet medical needs. This lab is used to perform cell-based studies like cytotoxicity, biocompatibility and various molecular and cellular assays for identifying the unique characteristics of a disease and help bridge the gap between identification of disease and application of therapy.

Lab In-charge Prof. Amit Kumar Jaiswal

Email: amitj@vit.ac.in

1. iMARK plate reader

2. Bio photometer

3. ChemiDoc-It² Imager

4. SDS-PAGE Setup

5. PCR Thermal cycler

6. qTOWER³ g (qPCR)

7. Trans-blot SD Semidry transfer cell

8. BSL 2 Biosafety Cabinet

9. CO2Incubator

10. Refrigerated Shaking incubator

11. Thermo Scientific Smart2Pure

12. Gel Rocker

13. Dry Heating Block

Tissue Engineering Lab

This facility has all the basic equipment’s needed to fabricate polymeric scaffolds, hydrogels and nanomaterials which are crucial for tissue engineering lab.

Lab In-charge Dr. Joseph Nathanael A

Email: joseph.nathanael@vit.ac.in

1. CO2Incubator

2. REMI Refrigerated Centrifuge

3. Deep Freezer (-20)

4. Infinite® 200 PRO M plex Plate Reader (Tecan)

5. Nanoparticle Tracking Analysis (NTA) Zeta View

6. Alpha 1-2 Ldplus Freeze Dryer (Lyophilization)

7. Electrospinning Machine

8. Cryo Scientific Ultra Freeze

9. Water Bath

10. BSL 2 Biosafety Cabinet

Antimicrobial Studies Laboratory

This laboratory has all the basic equipment’s needed to perform anti-microbial studies.

Lab in-charge: Dr. Raunak Kumar Das

Email: raunakkumardas@vit.ac.in

1. Biosafety Cabinet

2. Orbital Shaker Incubator

Precision Nano Medicine Lab (PNML) & Micro Fluidics and Fabrication Facility

Lab In-charge: Dr. Arunkumar Pitchaimani

Email: arunkumar.pitchaimani@vit.ac.in

1. Clean Room Biosafety Cabinet

2. Smart Print UV (Lithography Unit)

3. ELEGOO (Resin 3D Printer)

4. Analytical Balance

5. Spin Coater

6. Digital Ultrasonic Cleaner

7. Stereomicroscope

8. Hot Plate Magnetic Stirrer

9. Hot Air Oven

10. Syringre Pump

11. Microfluidic Flow Controller

12. Corona Treater

13. Vaccum Pump

14. Compressor Pump

Bio translational Medicine Laboratory

Lab In-charge: Dr. Sushma Kumari and Dr. U. Anjaneyulu

Email: sushma.kumari@vit.ac.in , Anjaneyulu.u@vit.ac.in

1. Harrick Plasma – Basic Plasma Cleaner

2. Pelletizer (Hydraulic Press)

3. REMI – Homogenizer

4. Oil Bath with Magnetic Stirrer

5. Tarsons Magnetic Stirrer and hot plate

6. Abdos Magnetic stirrer with hot plate

7. Contech - pH Meter

8. REMI Hotplate with magnetic stirrer

9. Borosil – Hot plate with magnetic stirrer

Bio-Mechanics Lab Facility

Lab In-charge: Dr. D S Mohan Varma

Email: mohanvarma@vit.ac.in

Gait Rehabilitation Device

Bioprocessing Lab & Biocorrosion and Bio Tribology Lab

Lab In-charge: Prof. Geetha Manivasagam and Dr. Loganathan Rangasamy

Email: geethamanivasagam@vit.ac.in and loganathan.r@vit.ac.in

1. Scratch Tester Machine – Ducom TR 104

2. Box furnace (1200 °C) – VB Ceramics

3. Box Furnace (1200 oC) – VB Ceramics

4. Fretting Wear Tester - Ducom

5. Corrosion Testing Setup with 3-lead potentiostat

6. Contact Angle Reader - Holmarc

7. Vickers Microhardness Tester – Chennai Metco

8. Upright Microscope - Carl ZEISS Axio

9. Reciprocatory Wear Tester - Ducom

10. Mammalian Cell Culture Facility for Biomaterial Testing

11. UV-Vis Spectrophotometer – Gensys 180

12. Tubular Furnace (1400 o C) – VB Ceramics

13. Hot mounting machine – Chennai Metco

14. Tabletop Double Disc Polishing Machine – Bainpol Chennai Metco

15. Anodization Setup

16.Ultrasonicator - Labman

17. Magnetic Stirrer With Hot plate - LabQuest

18. Precision Weighing Balance - Shimadzu

19. -20 Freezer – Cryo Scientific

20. pH Meter - Contech

21. Low Speed Saw – Baincut Chennai Metco

22. Sterilization Autoclave -Technico

23. Hote Air Oven – VB Ceramics

24. Polishing Machine – Bainpol Chennai Metco

Smart Biomaterials Lab

Lab In-charge: Dr. A. Joseph Nathanael

Email: joseph.nathanael@vit.ac.in

1. Fume hood - Best lab

2. Hot air oven (small) - Loyal instruments

3. Linear vacuum oven- Linear enterprises

4. Automatic film applicator - HFPS delta

5. Digital ultrasonic cleaner (5 L) - Kinglab

6. Digital ultrasonic cleaner (24 L) - Labman

7. Multi magnetic stirrer digital (5) positions - Toplab

8. Digital magnetic stirrer with hot plate - ABDOS

9. Digital magnetic stirrer with hot plate - REMI

10. Digital magnetic stirrer with hot plate - DAN

11. Analytical balance - Essae

12. Bullet Ice cube machine- Bluestar

Other Facilities

Vortex

pH Meter

Magnetic Stirrer

3600 Tube Rotator

Micro Oven

Heating Mantel

UV Cabinet

Spin Win Centrifuge

Micro Balance

Liquid Nitrogen Cylinder

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